اثر حفاظتی عصاره هیدروالکلی دانه گیاه سیاهدانه بر سمیت کبدی القا شده با جنتامایسین درموش‌های صحرایی نر

Background: Gentamicin is an aminoglycosides antibiotic which is used to treat several types of bacterial infections. The aim of this study was to evaluate the effects of Nigella sativa L. extract (NSE) on liver toxicity and serum level of liver enzymes in rats induced by gentamicin. Methods: In the present experimental study, 42 Wistar male rats were divided randomly into 6 groups includes: control (normal saline 2ml/kg, i.p), gentamicin (gentamicin 80mg/kg/day, i.p), positive control (400mg/kg NSE, i.p) and treated groups 1, 2, 3 (gentamicin 80mg/kg/day+ NSE 200, 400 and 800 mg/kg respectively, i.p). Nigella sativa extract was injected for 7 days after ten days period of gentamicin injection. After the examination the blood samples were collected from heart directly and the serum of ALT, AST and ALP liver enzymes were measured. Microscopic studies of hepatic tissue were done. All data analyzed with ANOVA and Tukey test. Results: Gentamicin caused necrosis and inflammation in the liver tissue and significantly increased serum level of liver enzymes (p<0.001).Treatment with NSE significantly decreased serum level of liver enzymes in treated groups compared with the group receiving gentamicin (p<0.001).The greatest impact was created in high dose of NSE. Conclusion: Gentamicin can cause necrosis and inflammation effect in liver tissue. The Nigella sativa seed extract have antioxidant and flavonoid compounds that can decrease toxic effect of gentamicin in liver tissue. &nbsp;